Coding

Part:BBa_K3624000:Design

Designed by: Emily Hagens   Group: iGEM20_Lethbridge   (2020-10-25)


BMAP-18: Antimicrobial peptide.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 82
    Illegal PstI site found at 96
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 82
    Illegal PstI site found at 96
    Illegal NotI site found at 7
    Illegal NotI site found at 89
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 82
    Illegal PstI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 60


Design Notes

The amino acid sequence was codon-optimized.


Source

BMAP-18 originally comes from cows (Bos taurus).

AMP Database ID: AP00366

References

Mardirossian, M., Pompilio, A., Degasperi, M., Runti, G., Pacor, S., Bonaventura, G. D., & Scocchi, M. (2017). D-BMAP18 Antimicrobial Peptide Is Active In vitro, Resists to Pulmonary Proteases but Loses Its Activity in a Murine Model of Pseudomonas aeruginosa Lung Infection. Frontiers in Chemistry, 5. doi:10.3389/fchem.2017.00040

Sungtae Yang, Chul Won Lee, Hak Jun Kim, Hyun-Ho Jung, Jae Il Kim, Song Yub Shin, Sung-Heui Shin, Structural analysis and mode of action of BMAP-27, a cathelicidin-derived antimicrobial peptide, Peptides, Volume 118, 2019, 170106, ISSN 0196-9781, https://doi.org/10.1016/j.peptides.2019.170106.

Guizi Ye, Hongyu Wu, Jinjiang Huang, Wei Wang, Kuikui Ge, Guodong Li, Jiang Zhong, Qingshan Huang, LAMP2: a major update of the database linking antimicrobial peptides, Database, Volume 2020, 2020, baaa061, https://doi.org/10.1093/database/baaa061